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My Projects

Project Name Submission Time Sequence Count Status Actions

Submit New Prediction

Select Model
PlaszymeAlpha
PlaszymeAlpha
Sequence-based ML model for fast prioritization and validation.
PlaszymeX
Structure-aware GNN model for advanced prediction and novel plastics.

Due to uncontrollable network fluctuations during data packet parsing on the MSA server, ColabFold is temporarily unable to return structural information.

Enter protein sequences in FASTA format (up to 20 sequences)
Please enter valid protein sequences
or

Drag and drop FASTA file here or click to upload

Supported formats: .fasta, .fa, .txt (Max: 10MB)

or
Upload a PDB file corresponding to your sequence (single chain, residue-level recommended). Providing a structure will bypass automatic prediction and generally improves accuracy and efficiency.

Click to upload or drag and drop file here

Supported formats: .pdb, .ent (Max: 50MB)

After submission, please return to Dashboard and manually refresh your browser to view the status.

Please enter a project name

Metagenome Analysis

Upload raw metagenomic data for comprehensive plastic degradation enzyme discovery. The system will automatically perform gene prediction, protein extraction, and batch analysis with optimized parameters.

Upload Metagenome Data

Drag and drop your metagenomic files here or click to select

Supported: FASTA, FASTQ, compressed files (.gz, .zip)

Maximum file size: 500MB per file

Processing Time: Metagenome analysis typically takes 30-120 minutes depending on data size. You'll receive an email notification when completed.

After submission, please return to Dashboard and manually refresh your browser to view the status.

Historical Projects

Project Name Submission Time Sequence Count Status Actions
Marine Microbial Sample 2025-08-28 14:30 5 Complete
Soil Sample Test 2025-08-27 09:15 12 Complete

Marine Microbial Sample

Submission time: 2025-08-28 14:30 | 5 sequences | Confidence: High

3D Structure Viewer

Interactive protein structure will be displayed here

Due to uncontrollable network fluctuations during data packet parsing on the MSA server, ColabFold is temporarily unable to return structural information.

Input Sequence Information

Sequence ID Length Sequence Fragment

Prediction Results

Sequence ID Plastic Type Degradation Probability Confidence

About Plaszyme

Plaszyme is an online platform dedicated to predicting plastic-degrading enzymes. Utilizing advanced machine learning models (including algorithms trained on sequence patterns) and cutting-edge structure-based deep learning models, the platform analyzes protein sequences to predict their ability to degrade various types of plastics. This platform aims to provide convenient tools for biotechnology researchers, environmental scientists, and industry professionals to accelerate plastic degradation research and applications.

Our prediction capability is robust: the models are trained on extensive experimentally validated plastic-degrading enzyme data (PlaszymeDB), accurately identifying both sequence patterns and structural features critical for plastic degradation.

The platform now primarily offers prediction functions through PlaszymeAlpha and PlaszymeX. Users have the flexibility to select their preferred model based on the task mode or utilize the metagenomic prediction mode to process environmental samples. All underlying models are rigorously trained on PlaszymeDB data.

Key Features:

  • Support for degradation prediction of multiple common plastic, total 34 types (PET, PS, PP, PE, PLA, PCL, PHA, etc.)
  • Multiple models and task modes are provided.
  • Intuitive result display and data export functions (CSV, PDF)
  • Browser-based local storage project management feature, no registration required
  • Detailed usage tutorials and technical support

Usage Tutorial

1

Select Prediction Mode and Model

Before preparing your data, first select the appropriate analysis path based on your research goals:

Standard Prediction Mode (PlaszymeAlpha / PlaszymeX): For analyzing individual or small batches of protein sequences. You must choose one of the core models (PlaszymeAlpha or PlaszymeX) based on your preference for speed or in-depth analysis (e.g., structure-based prediction).

Metagenomic Prediction Mode: For processing large environmental or uncurated protein sequence datasets. This mode utilizes specialized algorithms optimized for high-throughput, environmental screening.

2

Prepare Protein Sequences

Prepare the protein sequences you want to analyze. Ensure sequences are properly formatted with unique identifiers.

Format Supported: Supports single sequence text input or batch sequences in FASTA format files (.fasta, .fa, .txt).

3

Submit Prediction Task

Navigate to the "Predict" page.

Enter an optional project name.

Paste your sequences in the text box or drag and drop your FASTA files into the upload area.

Confirm your chosen Prediction Mode and Model selection (from Step 1) on the submission interface.

Click the "Submit Prediction" button to start the analysis.

4

Wait for Processing Completion

The system will start processing your sequences. Processing time depends on the chosen model (Standard vs. Metagenomic Mode) and the number/length of the sequences. After processing is complete, a "View Report" button will appear.

5

View and Export Results

On the report page, you can view the detailed prediction results.

Data Display: Results show each sequence's degradation probability against different plastic types, displayed in intuitive progress bars with confidence annotations.

Export Options: You can export the full results in CSV or PDF format for further analysis or reporting.